Supreme Court judge Bryan Sykes has turned down the application to accept two witness statements at the murder trial of dancehall artiste Vybz Kartel.
Justice Sykes handed down his ruling a short while ago.
He said he was not convinced that the prosecution took enough steps to locate the witnesses.
Justice Sykes says the jury will now be directed to return a formal verdict of not guilty.
The 12-member jury is to return to the Home Circuit Court tomorrow.
This now means that Kartel, whose given name is Adidja Palmer, will be acquitted.
He and Lenburgh McDonald and Nigel Thompson were taken into custody for the 2011 shooting murder of the St Catherine businessman, Barrington 'Bossy' Burton.
However he will remain in custody because he has another murder case pending against him.